Publications by Category

Antibody Engineering and the MHC Class I-Related Receptor, FcRn

  • Swiercz, R., Chiguru, S., Tahmasbi, A., Ramezani, S. M., Hao, G., Challa, D. K., Lewis, M. A., Kulkarni, P. V., Sun, X., Ober, R. J., Mason, R. P., and Ward, E. S.(2014) Use of Fc-engineered antibodies as clearing agents to increase contrast during PET. J. Nucl. Med.551204—1207.
  • Kang, J. C., Poovassery, J. S., Bansal, P., You, S., Manjarres, I. M., Ober, R. J., and Ward, E. S. (2013) Engineering multivalent antibodies to target heregulin-induced HER3 signaling in breast cancer cells. mAbs6340—353.
  • Devanaboyina, S., Lynch, S. M., Ober, R. J., Ram, S., Kim, D., Puig-Canto, A., Breen, S., Kasturirangan, S., Fowler, S., Peng, L., Zhong, H., Jermutus, L., Wu, H., Webster, C., Ward, E. S., and Gao, C. (2013) The effect of pH dependence of antibody-antigen interactions on subcellular trafficking dynamics. mAbs5849—857.
  • Gan, Z., Ram, S., Ober, R. J., and Ward, E. S. (2013) Using multifocal plane microscopy to reveal novel trafficking processes on the recycling pathway. J. Cell Sci.1261176—1188.
  • Patel, D. A., Puig-Canto, A., Challa, D. K., Montoyo H. P., Ober, R. J., and Ward, E. S. (2011) Neonatal Fc receptor blockade by Fc engineering ameliorates arthritis in a murine model, J. Immunol.1871015—1022.
  • Montoyo, HP., Vaccaro, C., Hafner, M., Ober, R. J., Mueller, W., and Ward, E. S.(2009) Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice, Proc. Natl. Acad. Sci. USA1062788—2793.
  • Gan, Z., Ram, S., Vaccaro, C., Ober, R. J., and Ward, E. S. (2009) Analyses of the recycling receptor, FcRn, in live cells reveal novel pathways for lysosomal delivery, Traffic10600—614.
  • Mi, W., Wanjie, S., Lo, S. T., Gan, Z., Pickl-Herk, B., Ober, R. J., and Ward, E. S.(2008) Targeting the neonatal Fc receptor for antigen delivery using engineered Fc fragmentsJ. Immunol.1817550—7561.
  • Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J. and Ward, E. S. (2007) Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy,Proc. Natl. Acad. Sci. USA1045889—5894.
  • Vaccaro, C., Bawdon, R., Wanjie, S., Ober, R. J. and Ward, E. S. (2006)Divergent activities of an engineered antibody in murine and human systems have implications for therapeutic antibodies, Proc. Natl. Acad. Sci. USA10318709—18714.
  • Vaccaro, C., Zhou, J., Ober, R. J. and Ward, E. S. (2005) Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels, Nature Biotech.231283—1288.
  • Zhou, J., Mateos, F., Ober, R. J. and Ward, E. S. (2005) Conferring the binding properties of the mouse MHC Class I-related receptor, FcRn, onto the human ortholog by sequential rounds of site-directed mutagenesis, J. Mol. Biol.3451071—1081.
  • Ober, R. J., Martinez, C., Lai, X., Zhou, J. and Ward, E. S. (2004) Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level, Proc. Natl. Acad. Sci. USA10111076—11081.
  • Ober, R. J., Martinez, C., Vaccaro, C., Zhou, J., and Ward, E. S. (2004) Visualizing the site and dynamics of IgG salvage by the MHC Class I-related receptor, FcRn, J. Immunol.1722021—2029.
  • Zhuo, J., Johnson, J., Ghetie, V., Ober, R. J. and Ward, E. S. (2003) Generation of mutated variants of the human form of the MHC class I-related receptor, FcRn, with increased affinity for mouse immunoglobulin G, J. Mol. Biol.332901—913.
  • Kim, J-K., Firan, M., Radu, C., Kim, C-H., Ghetie, V. and Ward, E. S. (1999)Mapping of the site on human IgG1 for binding of the MHC class I related receptor, FcRn, Eur. J. Immunol.292819—2825.
  • Ghetie, V., Popov, S., Borvak, J., Radu, C., Matesoi, D., Medesan, C., Ober, R. J. and Ward, E. S. (1997) Increasing the serum persistence of an IgG fragment by random mutagenesis, Nature Biotech.15637—640.
  • Medesan, C., Matesoi, D., Radu, C., Ghetie, V. and Ward, E. S. (1997)Delineation of the amino acid residues involved in transcytosis and catabolism of mouse IgG, J. Immunol.1582211—2217.
  • Ghetie, V., Hubbard, J. G., Kim, J-K., Tsen, M-F., Lee, Y. and Ward, E. S. (1996)Abnormally short serum half lives of IgGs in beta2-microglobulin deficient mice, Eur. J. Immunol.26690—696.
  • Ward, E.S., Güssow, D., Griffiths, A., Jones, P.T. and Winter, G. (1989) Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli, Nature341544—546.

Autoimmune Disease and T Cell Biology

  • Challa, D. K., Bussmeyer, U., Khan, T., Montoyo, H. P., Bansal, P., Ober, R. J., and Ward, E. S. (2013) Autoantibody depletion ameliorates disease in murine experimental autoimmune encephalomyelitis. mAbs5655—659.
  • Bansal, P., Khan, T., Bussmeyer, U., Challa, D. K., Swiercz, R., Velmurugan, R., Ober, R. J., and Ward, E. S. (2013) The encephalitogenic, human myelin oligodendrocyte glycoprotein-induced antibody repertoire is directed toward multiple epitopes in C57BL/6 mice. J. Immunol.1911091—1101.
  • Maverakis, E., Menezes, J. S., Ametani, A., Han, M., Stevens, D. B., He, Y., Wang, Y., Ono, Y., Miyamura, Y., Lam, K. S., Ward, E. S., and Sercarz, E. E.,(2010) Molecular mimics can induce a nonautoaggressive repertoire that preempts induction of autoimmunity, Proc. Natl. Acad. Sci. USA1072550—2555.
  • Pastor, S., Minguela, A., Mi, W., and Ward, E. S. (2009) Autoantigen immunization at different sites reveals a role for anti-inflammatory effects of IFN-gamma in regulating susceptibility to experimental autoimmune encephalomyelitisJ. Immunol.1825268—5275.
  • Minguela, A., Pastor, S., Mi, W., Richardson, J. A., and Ward, E. S. (2007)Feedback regulation of murine autoimmunity via dominant anti-inflammatory effects of interferon gammaJ. Immunol.178134—144.
  • Huang, J. C., Ober, R. J. and Ward, E. S. (2005) The central residues of a T cell receptor sequence motif are key determinants of autoantigen recognition in murine experimental autoimmune encephalomyelitisEur. J. Immunol.35299—304.
  • Huang, J. C., Han, M., Minguela, A., Pastor, S., Qadri, A. and Ward, E.S. (2003) T cell recognition of distinct peptide: I-Au conformers in murine experimental autoimmune encephalomyelitisJ. Immunol.1712467—2477.
  • Machius, M., Cianga, P., Deisenhofer, J., and Ward, E. S. (2001) Crystal structure of a T cell receptor Va11 (AV11S5) domain: new canonical forms for the first and second complementarity determining regionsJ. Mol. Biol.310689—698.
  • Garcia, K. C., Radu, C. G., Ho, J., Ober, R. J., and Ward, E. S. (2001) Kinetics and thermodynamics of T cell receptor-autoantigen interactions in murine experimental autoimmune encephalomyelitisProc. Natl. Acad. Sci. USA986818—6823.
  • Qadri, A., Radu, C. G., Thatte, J., Cianga, P., Ober, B. T., Ober, R. J., and Ward, E. S. (2000) A role for the region encompassing the c” strand of a T cell receptor Va domain in T cell activation eventsJ. Immunol.165820—829.
  • Thatte, J., Qadri, A., Radu, C., and Ward, E. S. (1999) Molecular requirements for T cell recognition by an MHC Class II restricted T cell receptor: the involvement of the fourth hypervariable loop of the Va domainJ. Exp. Med.,189509—519.
  • Fields, B. A., Ober, B., Malchiodi, E. L., Lebedeva, M. I., Braden, B., Ysern, X., Kim, J-K., Shao, X., Ward, E. S., and Mariuzza, R. A. (1995) Crystal structure of the Va domain of a T cell antigen receptorScience2701821—1824.

Fluorescence Microscopy

  • Tahmasbi, A., Ram, S., Chao, J., Abraham, A. V., Tang, F. W., Ward, E. S., and Ober, R. J. (2014) Designing the focal plane spacing for multifocal plane microscopy. Opt. Express2216706—16721.
  • Chao, J., Ram, S., Ward, E. S., and Ober, R. J. (2013) Ultrahigh accuracy imaging modality for super-localization microscopy. Nat. Methods10335—338.
  • Gan, Z., Ram, S., Ober, R. J., and Ward, E. S. (2013) Using multifocal plane microscopy to reveal novel trafficking processes on the recycling pathway. J. Cell Sci.1261176—1188.
  • Ram, S., Kim, D., Ward, E. S., and Ober, R. J. (2012) 3D single molecule tracking with multifocal plane microscopy reveals rapid intercellular transferrin transport at epithelial cell barriers. Biophys. J.103(7)1594-1603.
  • Ram, S., Prabhat, P., Chao, J., Ward, E. S., and Ober, R. J. (2010) Three-dimensional Single molecule tracking of quantum-dot labeled antibody moleculesProc. SPIE.757575750J.
  • Abraham, A. V., Ram, S., Chao, J., Ward, E. S., and Ober, R. J. (2009)Quantitative study of single molecule location estimation techniquesOpt. Express1723352—23373.
  • Ram, S., Prabhat, P., Ward, E. S., and Ober, R. J. (2009) Improved single particle localization accuracy with dual objective multifocal plane microscopy,Opt. Express176881—6898.
  • Chao, J., Ram, S., Abraham, A. V., Ward, E. S., and Ober, R. J. (2009) A resolution measure for three-dimensional microscopyOptics Com.2821751—1761.
  • Ram, S., Prabhat, P., Chao, J., Ward, E. S., and Ober, R. J. (2008) High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cellsBiophys. J.956025—6043.
  • Prabhat, P., Gan, Z., Chao, J., Ram, S., Vaccaro, C., Gibbons, S., Ober, R. J., and Ward, E. S. (2007) Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy,,Proc. Natl. Acad. Sci. USA1045889—5894.
  • Prabhat, P., Ram, S., Ward, E. S., and Ober, R. J. (2006) Simultaneous imaging of several focal planes in fluorescence microscopy for the study of cellular dynamics in 3DProc. SPIE.6090115—121.
  • Ram, S., Ward, E. S., and Ober, R. J. (2006) Beyond Rayleigh’s criterion: A resolution measure with application to single-molecule microscopyProc. Natl. Acad. Sci. USA1034457—4462.
  • Lai, X., Lin Z., Ward, E. S., and Ober R. J. (2005) Noise suppression of point spread functions and its influence on deconvolution of three dimensional fluorescence microscopy image setsJ. Microscopy21793—108.
  • Ober, R. J., Martinez, C., Lai, X., Zhou, J., and Ward, E. S. (2004) Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule levelProc. Natl. Acad. Sci. USA10111076—11081.
  • Prabhat, P., Ram, S., Ward, E. S., and Ober, R. J. (2004) Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions.IEEE Trans. Nanobio.3237—242.
  • Ober, R. J., Ram, S., and Ward, E. S. (2004) Localization accuracy in single-molecule microscopyBiophys. J.861185—1200. Please see MIATool for more information.

Signal Processing

  • Cohen, E. A. K. and Ober, R. J. (2013) Analysis of point based image registration errors with applications in single molecule microscopyIEEE Trans. Signal Process.616291—6306.
  • Ram, S., Ward, E. S., and Ober, R. J. (2013) A stochastic analysis of distance estimation approaches in single molecule microscopy – quantifying the resolution limits of photon-limited imaging systemsMultidim. Sys. Sig. Proc.,24503—542.
  • Chao, J., Ward, E. S., and Ober, R. J. (2012) Fisher information matrix for branching processes with application to electron-multiplying charge-coupled devicesMultidim. Sys. Sig. Proc.23349—379.
  • Wong, Y., Lin, Z., and Ober, R. J. (2011) Limit of the accuracy of parameter estimation for moving single molecules imaged by fluorescence microscopy,IEEE Trans. Sig. Proc.59895—911.
  • Chao, J., Ward, E. S., and Ober, R. J. (2010) A software framework for the analysis of complex microscopy image dataIEEE Trans. Info. Tech. Biomed.,141075—1087.
  • Abraham, A. V., Ram, S., Chao, J., Ward, E. S., and Ober, R. J. (2010)Comparison of estimation algorithms in single-molecule localizationProc. SPIE.7570757004.
  • Zou, Q., Lin, Z., and Ober, R. J. (2006) The Cramer Rao lower bound for bilinear systemsIEEE Trans. Sig. Proc.541666—1680.
  • Ram, S., Ward, E. S., and Ober, R. J. (2006) A stochastic analysis of performance limits for optical microscopesMultidim. Sys. Sig. Proc.1527—57.
  • Lin, Z., Zou, Q., Ward, E. S. and Ober, R. J. (2005) The Cramer Rao lower bound for parameter estimation in nonlinear systemsIEEE Sig. Proc. Letts.,12855—858.
  • Ober, R. J., Lai, X., Lin, Z. and Ward, E. S. (2005) State space realization of a three-dimensional image set with application to noise reduction in fluorescent microscopy images of cellsMultidim. Sys. Sig. Proc.167—48.
  • Hunt, L. R., Ward, E. S., and Ober, R. J. (2004) Approximation of trajectories of nonlinear systems by iterates of systems with linear state dynamicsSys. Cont. Letts.51377—381.
  • Ober, R. J., Lin, Z., and Zou, Q. (2003) Calculations of the Fisher information matrix for multidimensional data setsIEEE Trans. on Sig. Proc.51(10)2679—2691.
  • Ober, R. J., Lin, Z., Ye, H., and Ward, E. S. (2002) Achievable accuracy of parameter estimation for multidimensional NMR experimentsJ. Mag. Reson.1571—16.
  • Ober, R. J. (2002) The Fisher information matrix for linear systemsSys. Contr. Letts.47221—226.

Surface Plasmon Resonance (SPR)

  • Ober, R. J., Caves, J., and Ward, E. S. (2003) Analysis of exponential data using a non-iterative technique: application to surface plasmon experimentsAnal. Biochem.31257—65.
  • Ober, R. J. and Ward, E. S. (2002) Compensation for loss of ligand activity in surface plasmon resonance experimentsAnal. Biochem.306228—236.
  • Ober, R. J. and Ward, E. S. (1999) The influence of signal noise on the accuracy of kinetic constants measured by surface plasmon resonance experiments.Anal. Biochem.27170—80. Please see SPRTool for more information.